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cpe reduction assay  (Promega)

 
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    Promega cpe reduction assay
    Optimization of a <t>CPE-reduction</t> antiviral assay. ( A ) Percentage CPE and ( B ) Z’ value per MAYV inoculum in different cell lines (Vero, BHK, Huh-7, CRL2522 and A549 cells). Results were obtained with the MTS/PMS read-out method. Mean values ± standard deviations (SD) are shown of 3 independent experiments. CPE, cytopathogenic effect; PFU, plaque-forming unit. ( C ) Z’ value for three different MAYV <t>inocula</t> <t>(MOI</t> 0.01, 0.005, 0.001) on Vero cells. Data are presented as box plots showing individual and mean values of four independent experiments. MOI, multiplicity of infection.
    Cpe Reduction Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cpe reduction assay/product/Promega
    Average 90 stars, based on 1 article reviews
    cpe reduction assay - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Repurposing Drugs for Mayaro Virus: Identification of EIDD-1931, Favipiravir and Suramin as Mayaro Virus Inhibitors"

    Article Title: Repurposing Drugs for Mayaro Virus: Identification of EIDD-1931, Favipiravir and Suramin as Mayaro Virus Inhibitors

    Journal: Microorganisms

    doi: 10.3390/microorganisms9040734

    Optimization of a CPE-reduction antiviral assay. ( A ) Percentage CPE and ( B ) Z’ value per MAYV inoculum in different cell lines (Vero, BHK, Huh-7, CRL2522 and A549 cells). Results were obtained with the MTS/PMS read-out method. Mean values ± standard deviations (SD) are shown of 3 independent experiments. CPE, cytopathogenic effect; PFU, plaque-forming unit. ( C ) Z’ value for three different MAYV inocula (MOI 0.01, 0.005, 0.001) on Vero cells. Data are presented as box plots showing individual and mean values of four independent experiments. MOI, multiplicity of infection.
    Figure Legend Snippet: Optimization of a CPE-reduction antiviral assay. ( A ) Percentage CPE and ( B ) Z’ value per MAYV inoculum in different cell lines (Vero, BHK, Huh-7, CRL2522 and A549 cells). Results were obtained with the MTS/PMS read-out method. Mean values ± standard deviations (SD) are shown of 3 independent experiments. CPE, cytopathogenic effect; PFU, plaque-forming unit. ( C ) Z’ value for three different MAYV inocula (MOI 0.01, 0.005, 0.001) on Vero cells. Data are presented as box plots showing individual and mean values of four independent experiments. MOI, multiplicity of infection.

    Techniques Used: Antiviral Assay, Infection

    In vitro antiviral effect of EIDD-1931, favipiravir, suramin and ribavirin in Vero cells. ( A ) Dose–response effect of EIDD-1931, favipiravir, suramin and ribavirin on MAYV-induced CPE (MOI 0.01), quantified in Vero cells by the MTS/PMS method. Data shown are mean values ± standard deviations (SD) from three independent experiments. ( B – E ) The effect of different concentrations of ( B ) EIDD-1931, ( C ) favipiravir, ( D ) suramin and ( E ) ribavirin on the release of MAYV particles by infected Vero cells (MOI 0.01). Both viral RNA (genome copies/mL; blue) and infectious progeny virus (TCID 50 /mL; orange) were quantified at 48 h pi by real-time qRT-PCR and end-point titrations, respectively. Data shown are mean values ± SD from three independent experiments. Significant differences from untreated virus control were analyzed by Kruskal–Wallis test (*, p < 0.05; **, p < 0.005). CPE, cytopathogenic effect; TCID, tissue culture infectious dose; VC, virus control (untreated); LOD, limit of detection.
    Figure Legend Snippet: In vitro antiviral effect of EIDD-1931, favipiravir, suramin and ribavirin in Vero cells. ( A ) Dose–response effect of EIDD-1931, favipiravir, suramin and ribavirin on MAYV-induced CPE (MOI 0.01), quantified in Vero cells by the MTS/PMS method. Data shown are mean values ± standard deviations (SD) from three independent experiments. ( B – E ) The effect of different concentrations of ( B ) EIDD-1931, ( C ) favipiravir, ( D ) suramin and ( E ) ribavirin on the release of MAYV particles by infected Vero cells (MOI 0.01). Both viral RNA (genome copies/mL; blue) and infectious progeny virus (TCID 50 /mL; orange) were quantified at 48 h pi by real-time qRT-PCR and end-point titrations, respectively. Data shown are mean values ± SD from three independent experiments. Significant differences from untreated virus control were analyzed by Kruskal–Wallis test (*, p < 0.05; **, p < 0.005). CPE, cytopathogenic effect; TCID, tissue culture infectious dose; VC, virus control (untreated); LOD, limit of detection.

    Techniques Used: In Vitro, Infection, Quantitative RT-PCR



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    Optimization of a <t>CPE-reduction</t> antiviral assay. ( A ) Percentage CPE and ( B ) Z’ value per MAYV inoculum in different cell lines (Vero, BHK, Huh-7, CRL2522 and A549 cells). Results were obtained with the MTS/PMS read-out method. Mean values ± standard deviations (SD) are shown of 3 independent experiments. CPE, cytopathogenic effect; PFU, plaque-forming unit. ( C ) Z’ value for three different MAYV <t>inocula</t> <t>(MOI</t> 0.01, 0.005, 0.001) on Vero cells. Data are presented as box plots showing individual and mean values of four independent experiments. MOI, multiplicity of infection.
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    Image Search Results


    Overview of Experimental Conditions and Confirmation Assays Carried Out by Each of the Studies Reviewed

    Journal: Health Security

    Article Title: Lessons Learned From Limited Overlap of 15 In Vitro COVID-19 Drug Repurposing Screens

    doi: 10.1089/hs.2022.0132

    Figure Lengend Snippet: Overview of Experimental Conditions and Confirmation Assays Carried Out by Each of the Studies Reviewed

    Article Snippet: Mirabelli , Huh7 , SARS-CoV-2 USA-WA1/2020 , 0.2 , Cell morphology and SARS-CoV-2 immunostaining , 4 hours preinfection , 48 hours , Multi-cycle CPE reduction assay, synergy with remdesivir, validation in iAECs.

    Techniques: Infection, Incubation, Immunostaining, Activity Assay, Variant Assay, Enzyme-linked Immunosorbent Assay

    Optimization of a CPE-reduction antiviral assay. ( A ) Percentage CPE and ( B ) Z’ value per MAYV inoculum in different cell lines (Vero, BHK, Huh-7, CRL2522 and A549 cells). Results were obtained with the MTS/PMS read-out method. Mean values ± standard deviations (SD) are shown of 3 independent experiments. CPE, cytopathogenic effect; PFU, plaque-forming unit. ( C ) Z’ value for three different MAYV inocula (MOI 0.01, 0.005, 0.001) on Vero cells. Data are presented as box plots showing individual and mean values of four independent experiments. MOI, multiplicity of infection.

    Journal: Microorganisms

    Article Title: Repurposing Drugs for Mayaro Virus: Identification of EIDD-1931, Favipiravir and Suramin as Mayaro Virus Inhibitors

    doi: 10.3390/microorganisms9040734

    Figure Lengend Snippet: Optimization of a CPE-reduction antiviral assay. ( A ) Percentage CPE and ( B ) Z’ value per MAYV inoculum in different cell lines (Vero, BHK, Huh-7, CRL2522 and A549 cells). Results were obtained with the MTS/PMS read-out method. Mean values ± standard deviations (SD) are shown of 3 independent experiments. CPE, cytopathogenic effect; PFU, plaque-forming unit. ( C ) Z’ value for three different MAYV inocula (MOI 0.01, 0.005, 0.001) on Vero cells. Data are presented as box plots showing individual and mean values of four independent experiments. MOI, multiplicity of infection.

    Article Snippet: To determine the best cell line and the optimal MOI for the CPE reduction assay, cell viability was assessed using the MTS/PMS method as described by the manufacturer (Promega, Leiden, The Netherlands).

    Techniques: Antiviral Assay, Infection

    In vitro antiviral effect of EIDD-1931, favipiravir, suramin and ribavirin in Vero cells. ( A ) Dose–response effect of EIDD-1931, favipiravir, suramin and ribavirin on MAYV-induced CPE (MOI 0.01), quantified in Vero cells by the MTS/PMS method. Data shown are mean values ± standard deviations (SD) from three independent experiments. ( B – E ) The effect of different concentrations of ( B ) EIDD-1931, ( C ) favipiravir, ( D ) suramin and ( E ) ribavirin on the release of MAYV particles by infected Vero cells (MOI 0.01). Both viral RNA (genome copies/mL; blue) and infectious progeny virus (TCID 50 /mL; orange) were quantified at 48 h pi by real-time qRT-PCR and end-point titrations, respectively. Data shown are mean values ± SD from three independent experiments. Significant differences from untreated virus control were analyzed by Kruskal–Wallis test (*, p < 0.05; **, p < 0.005). CPE, cytopathogenic effect; TCID, tissue culture infectious dose; VC, virus control (untreated); LOD, limit of detection.

    Journal: Microorganisms

    Article Title: Repurposing Drugs for Mayaro Virus: Identification of EIDD-1931, Favipiravir and Suramin as Mayaro Virus Inhibitors

    doi: 10.3390/microorganisms9040734

    Figure Lengend Snippet: In vitro antiviral effect of EIDD-1931, favipiravir, suramin and ribavirin in Vero cells. ( A ) Dose–response effect of EIDD-1931, favipiravir, suramin and ribavirin on MAYV-induced CPE (MOI 0.01), quantified in Vero cells by the MTS/PMS method. Data shown are mean values ± standard deviations (SD) from three independent experiments. ( B – E ) The effect of different concentrations of ( B ) EIDD-1931, ( C ) favipiravir, ( D ) suramin and ( E ) ribavirin on the release of MAYV particles by infected Vero cells (MOI 0.01). Both viral RNA (genome copies/mL; blue) and infectious progeny virus (TCID 50 /mL; orange) were quantified at 48 h pi by real-time qRT-PCR and end-point titrations, respectively. Data shown are mean values ± SD from three independent experiments. Significant differences from untreated virus control were analyzed by Kruskal–Wallis test (*, p < 0.05; **, p < 0.005). CPE, cytopathogenic effect; TCID, tissue culture infectious dose; VC, virus control (untreated); LOD, limit of detection.

    Article Snippet: To determine the best cell line and the optimal MOI for the CPE reduction assay, cell viability was assessed using the MTS/PMS method as described by the manufacturer (Promega, Leiden, The Netherlands).

    Techniques: In Vitro, Infection, Quantitative RT-PCR